After doing a front brake service on Mrs B's R65LS, I spun the wheel to check the brakes were free & noticed that it didn't seem to be running true - long story short, there is a flat on the rim between two of the spokes (cast wheel). Don't know when this happened, it doesn't seem to affect handling or balance, but I suspect that this was done on (in ? by ?) a pothole. While the LS has cast wheels, they are two part, with the spokes & hub cast onto a forged (IIRC) rim & it runs with an inner tube - no loss of air noticed.
Given the force required to break the bead on a tubeless tyre to change it, plus the immediate & total loss of air when the bead is broken, I would think it unlikely that the pothole dislodged the tyre to give a delayed deflation. More likely sidewall or rim damage ? But only guesswork here, so maybe not !